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KMID : 0391319930030010031
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Korean Journal of Biological Response Modifiers
1993 Volume.3 No. 1 p.31 ~ p.38
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The Effects of Cytokines on IL-2 Induced NK Cell and LAK Cell Activity
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Abstract
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Immunotherapy using IL-2 and IL-2 induced LAK cells have induced some tumors to regress in patients with advanced cancers. But life threatening toxicities of IL-2 in dose escalation was themajor obstacle in clinical application because high dose
of
IL-2
is required to get effective anti-cancer activities. Varieties of cytokines including αinterferon, γ-interferon, Il-4 and even cyclophosphamide have been used in combination with IL-2 in an attempt to augment cytolytic activity of LAK cells and
to
reduce IL-2 dose with effective cytolytic activity, and with little harmful side reactions.
GM-CSF, α-interferon and γ-Interferon were added to a culture of human peripheral blood mononuclear cells in the absence and in he presence of IL-2 to elucidate their effects on the induction of LAK cells and the cytotoxicity of LAK cells in
vitro. NK
cell sensitive K-562 cells and NK cell-resistant Daudi cells were used to measure for NK cell and LAK cell activity using 4 hr-51Cr release assay from tumor cells, respectively.
For LAK cell induction, IL-2 was most effective one and then α-interferon the next. But γ-interferon and GM-CSF have little effect on it. For the augmentation of NK activity, IL-2 was also the best and α-interferon, GM-CSF and γ-interferon in
decreasing order. The combination of IL-2 with α-interferon and IL-2 with GM-CSF augumented the cytotoxicity of LAK cells. Like α-interferon which has been previously known, the substantial cytotoxic activity.
In this study auther conclude that the combination therapy with IL-2/GM-γCSF or IL-2/α-interferon as an adoptive immunotherapy would increase antitumor cytotoxic potentials and decrease IL-induced serious side-reactions.
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KEYWORD
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